Not known Facts About PP88
Not known Facts About PP88
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The vector in accordance with the creation, ideally included right into a shipping and delivery car, ideally a bacteriophage capsid, preferably comprises no more than 100 restriction websites. In a preferred embodiment, the vector according to the creation, if possible included in a delivery car, comprises not more than ten restriction web-sites.
reported vector, bacterial shipping and delivery auto or donor bacterial cell might be extra notably administered in the form of a pharmaceutical or cosmetic composition comprising said vector, bacterial shipping automobile or donor bacterial cell and 馬上試玩 a pharmaceutically acceptable carrier.
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It should be observed that as made use of herein, the singular forms “a”, “an” and “the” consist of plural referents unless the context Evidently dictates in any other case.
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Mimic peptides of particular curiosity are bacterial mimic peptides that are connected with automobile-immune ailments, for example These mentioned in Negi et al.
to unravel this difficulty, the existing inventors have herein made a fresh technique that ensures that DNA payloads the moment shipped in target bacteria simply cannot replicate inside the focus on microorganisms but still Categorical the gene(s) of desire in a stage that is sufficient to exert the envisioned end result on the germs or on the host, with no need to have of the antibiotic resistance collection marker around the DNA payload, and without the will need of a range move using an antibiotic.
261、细菌噬菌体可选自短尾噬菌体科(非限制性地比如以下属：fri1病毒、kp32病毒、kp34病毒、phikmv病毒、prado病毒、sp6病毒、t7病毒、cp1病毒、p68病毒、phi29病毒、nona33病毒、pocj病毒、tl2011病毒、bcep22病毒、bpp1病毒、cba41病毒、dfl12病毒、ea92病毒、epsilon15病毒、f116病毒、g7c病毒、jwalpha病毒、kf1病毒、kpp25病毒、lit1病毒、luz24病毒、luz7病毒、n4病毒、nonana病毒、p22病毒、website page病毒、phieco32病毒、prtb病毒、sp58病毒、una961病毒和vp5病毒)。
The creation of claimed molecule of fascination by claimed specific receiver bacterial mobile may well need the supply of a nucleic acid of curiosity which includes one or more type(s) of gene(s) or team(s) of genes. In particular, explained nucleic acid of curiosity could possibly be chosen with the group consisting of the gene encoding mentioned molecule of desire, in particular explained HMM, several genes encoding a protein advanced that's the molecule of desire, particularly the HMM, a gene or team of genes encoding enzyme(s) of a metabolic pathway bringing about the production of the molecule of desire, specifically on the HMM, a coding nucleic acid which can be the molecule of fascination, especially the HMM, and a non-coding nucleic acid which is the molecule of interest, specifically the HMM.
In preferred embodiments, the mimic peptide is from Proteobacteria or Firmicutes. Of unique interest would be the gene sequences encoding 24 intestine bacterial peptides discovered by Negi et al. with homology to 4 human peptides from reduced molecular fat phosphotyrosine protein phosphatase, Aldehyde dehydrogenase loved ones 3 member B1, Maleylacetoacetate isomerase and Uracil-DNA glycosylase.
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In a certain embodiment, explained plasmid comprises an antibiotic resistance marker. In an alternate embodiment, reported plasmid is devoid of antibiotic resistance marker.
140、根据本公开的感兴趣的核酸可包含编码cas蛋白的核酸序列。多种crispr酶可用于用作质粒上感兴趣的序列。在一些实施方案中，crispr酶为ii型crispr酶。在一些实施方案中，crispr酶催化dna切割。在一些其他实施方案中，crispr酶催化rna切割。优选地，crispr酶不造成双链断裂。在一些实施方案中，crispr酶造成单链断裂或切口。在一些实施方案中，crispr酶不会在dna或rna中造成任何断裂。在一个实施方案中，cas13-脱氨酶融合被用于对rna进行碱基编辑。
primary modifying makes it possible for introduction of insertions, deletions (indels) and 12 base-to-base conversions. key editing depends on the ability of a reverse transcriptase (RT), fused to the Cas nickase variant, to convert RNA sequence brought by a primary enhancing information RNA (pegRNA) into DNA on the nick internet site generated through the Cas protein. The DNA flap created from this method is then included or not from the focused DNA sequence.
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